International Publications

Article: Combined antibody and DNA detection for early diagnosis of leptospirosis after a disaster

Hiroko Iwasaki · Haorile Chagan-Yasutan · Prisca Susan A. Leano · Nobuo Koizumi · Chie Nakajima · Delsi Taurustiati · Firmanto Hanan · Talitha Lea Lacuesta · Yugo Ashino · Yasuhiko Suzuki · Nina G. Gloriani · Elizabeth Freda O. Telan · Toshio Hattori

Diagn Microbiol Infect Dis 01/2016

ABSTRACT: Early diagnosis based on laboratory confirmation is essential for managing leptospirosis. This study investigated the effectiveness of a novel method of detecting leptospirosis that combines measurement of anti-Leptospira antibodies by the microscopic agglutination test (MAT), enzyme-linked immunosorbent assay (ELISA), and immunochromatographic test (ICT) and leptospiral DNA by loop-mediated isothermal amplification (LAMP) and real-time PCR in plasma and two types of urine pellets. Of 113 suspected cases, 68.1%, 76.1%, and 60.2% were positive by MAT, ELISA, and ICT, respectively. Real-time PCR using DNA purified from urine pellets collected by low speed centrifugation yielded positive signals for patients in late acute as well as early phase who were positive by LAMP using plasma DNA or urine pellets. Among antibody-negative patients, 9.5% were positive by DNA detection. These findings indicate that the leptospirosis detection rate is increased by combining antibody and DNA detection, providing a new tool for timely diagnosis of infection.

Article: Elevated OPN, IP-10, and Neutrophilia in Loop-Mediated Isothermal Amplification Confirmed Tuberculosis Patients

Beata Shiratori · Susan Leano · Chie Nakajima · Haorile Chagan-Yasutan · Toshiro Niki · Yugo Ashino · Yasuhiko Suzuki · Elisabeth Telan · Toshio Hattori

Mediators of Inflammation Oct 2014

ABSTRACT: Tuberculosis (TB) is the second most common cause of death from infectious diseases and results in high socioeconomic losses to many countries. Proper diagnosis is the first step in TB eradication. To develop a rapid, simple, and accurate diagnostic TB test and to characterize the prevalence of Mycobacterium tuberculosis (MTB) genotypes and immune profiles of TB patients, a total of 37 TB patients and 30 healthy control (HC) from Metro Manila were enrolled. Loop-mediated isothermal amplification (LAMP) reliably detected MTB infection. Manila genotype was identified by spoligotyping method in all TB patients. Osteopontin (OPN), interferon- γ -induced protein 10 kDa (IP-10), and neutrophil counts were found to reflect the acute stage of MTB infection. The sensitivity and specificity were 94.6% and 93.3%, respectively, for both OPN and IP-10, and they were 83.8% and 78.6%, respectively, for neutrophils. The combination of OPN, IP-10, neutrophil count, IL-6, IL-8,

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